以1，3-二氨基硫脲、苯甲醛与噻唑-5-甲醛为原料，合成了一种新型不对称双席夫碱结构荧光探针N'-亚苄基-2-（噻唑-5-基亚甲基）肼基-1-硫代碳酰肼（L），并通过NMR、IR、ESI-MS和元素分析对其结构进行表征。在缓冲溶液（pH=7.0）中，探针L可以高选择性地荧光识别Zn²⁺，检测限为3.5×10^-7 mol/L，pH适用范围为5~10。当Zn²⁺与探针L配位时，生成1：2型配合物（L-Zn²⁺），在365 nm紫外灯照射下，由无色变成亮绿色强荧光，具有潜在的应用价值。

N'-benzylidene-2-(thiazole-5-methylidene) hydrazine-1-thiocarbonyl hydrazide (L),a novel asymmetric bis-Schiff base fluorescent probe,is synthesized by using 1,3-thiocarbohydrazide,benzaldehyde and thiazole-5-formaldehyde as starting materials,and its structure is characterized by NMR,IR,ESI-MS and elemental analysis.In an ethanol/tris (pH 7.0) buffer solution,the probe L can high-selectively recognize Zn²⁺ with fluorescence responding,the experimental results demonstrate that the detection limit is 3.5×10^-7 mol·L^-1,and the proper pH range is 5-10.When Zn²⁺ is coordinated with probe L,a 1:2 type complex (L-Zn²⁺) is formed and fluorescence color of the system changes from non-fluoresce to strong bright-green fluorescence under 365 nm UV lamp irradiation.